Studies on Hydrogen Exchange. III. Deuterium Exchange of Carbonyl Compounds in Alkaline Media
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منابع مشابه
Rapid Hydrogen-Deuterium Exchange in Liquid Droplets.
The rate of hydrogen-deuterium exchange (HDX) in aqueous droplets of phenethylamine has been determined with submillisecond temporal resolution by mass spectrometry using nanoelectrospray ionization with a theta-capillary. The average speed of the microdroplets is measured using microparticle image velocimetry. The droplet travel time is varied from 20 to 320 μs by changing the distance between...
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The single biggest problem with solution-phase H/D exchange as a mass spectrometric probe of surface exposure in a protein (or protein complex) is back-exchange of H for D after the initial H/D exchange has been quenched. Back-exchange results in loss of pertinent data and also greatly hampers data analysis. Previously, very fast, cold (0-4 degrees C) HPLC was performed to help reduce back-exch...
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Hydrogen/deuterium exchange mass spectrometric (H/DXMS) methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exchange behavior of proteins in their native microenvironment. Using this method, a differ...
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1 Bradbury, E. M., W. C. Price, and G. R. Wilkinson, J. Mol. Biol., 4, 39 (1962). 2 von Hippel, P. H., and G. Felsenfeld, Biochemistry, 3, 27 (1964). 3 Englander, S. W., Biochemistry, 2, 798 (1963). 4Englander, S. W., and J. J. Englander, these PROCEEDINGS, 53, 370 (1965). 5 Bray, G. A., Anal. Biochem., 1, 279 (1960). 6 Flodin, P., J. Chromatoq., 6, 103 (1961). 7 Doty, P., B. B. McGill, and S. ...
متن کاملDual Studies on a Hydrogen–Deuterium Exchange of Resorcinol and the Subsequent Kinetic Isotope Effect
An efficient laboratory experiment has been developed for undergraduate students to conduct hydrogen-deuterium (H-D) exchange of resorcinol by electrophilic aromatic substitution using D2O and a catalytic amount of H2SO4. The resulting labeled product is characterized by 1H NMR. Students also visualize a significant kinetic isotope effect (kH/kD ≈ 3 to 4) by adding iodine tincture to solutions ...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1966
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.14.1413